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Astragalus Extract Attenuates Allergic Airway Inflammation and Inhibits Nuclear Factor κB Expression in Asthmatic Mice

      Abstract

      Background

      Astragalus membranaceus from traditional Chinese herbal medicines previously showed that it possesses a strong anti-inflammatory activity. The purpose of this study was to elucidate the effect of astragalus on allergen-induced airway inflammation and airway hyperresponsiveness and investigate its possible molecular mechanisms.

      Methods

      Female BALB/c mice sensitized and challenged with ovalbumin (OVA) developed airway inflammation. Bronchoalveolar lavage fluid was assessed for total and differential cell counts and cytokine and chemokine levels. In vivo airway responsiveness to increasing concentrations of methacholine was measured 24 hours after the last OVA challenge using whole-body plethysmography. The expression of inhibitory κB-α and p65 in lung tissues was measured by Western blotting.

      Results

      Astragalus extract attenuated lung inflammation, goblet cell hyperplasia and airway hyperresponsiveness in OVA-induced asthma and decreased eosinophils and lymphocytes in bronchoalveolar lavage fluid. In addition, astragalus extract treatment reduced expression of the key initiators of allergic Th2-associated cytokines (interleukin 4, interleukin 5) (P<0.05). Furthermore, astragalus extract could inhibit nuclear factor κB (NF-κB) expression and suppress NF-κB translocation from the cytoplasm to the nucleus in lung tissue samples.

      Conclusions

      Taken together, our current study demonstrated a potential therapeutic value of astragalus extract in the treatment of asthma and it may act by inhibiting the expression of the NF-κB pathway.

      Key Indexing Terms

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      REFERENCES

        • Di Giampaolo L.
        • Quecchia C.
        • Schiavone C.
        • et al.
        Environmental pollution and asthma.
        Int J Immunopathol Pharmacol. 2011; 24: 31-38
        • de Diego Damiá A.
        • Vega Chicote J.M.
        Therapeutic approach to the distal airways in asthma [in Spanish].
        Arch Bronconeumol. 2011; 47: 27-31
        • Hart L.
        • Lim S.
        • Adcock I.
        • et al.
        Effects of inhaled corticosteroid therapy on expression and DNA-binding activity of nuclear factor κB in asthma.
        Am J Respir Crit Care Med. 2000; 161: 224-231
        • Chen F.
        • Castranova V.
        • Li Z.
        • et al.
        Inhibitor of nuclear factor kappaB kinase deficiency enhances oxidative stress and prolongs c-Jun NH2-terminal kinase activation induced by arsenic.
        Cancer Res. 2003; 63: 7689-7693
        • Yang G.
        • Abate A.
        • George A.G.
        • et al.
        Maturational differences in lung NF-κB activation and their role in tolerance to hyperoxia.
        J Clin Invest. 2004; 114: 669-678
        • Cui X.F.
        • Imaizumi T.
        • Yoshida H.
        • et al.
        Lipopolysaccharide induces the expression of cellular inhibitor of apoptosis protein-2 in human macrophages.
        Biochim Biophys Acta. 2000; 1524: 178-182
        • Na D.
        • Liu F.N.
        • Miao Z.F.
        • et al.
        Astragalus extract inhibits destruction of gastric cancer cells to mesothelial cells by anti-apoptosis.
        World J Gastroenterol. 2009; 15: 570-577
        • Zhang L.
        • Yang Y.
        • Wang Y.
        • et al.
        Astragalus membranaceus extract promotes neovascularisation by VEGF pathway in rat model of ischemic injury.
        Pharmazie. 2011; 66: 144-150
        • Qu Z.H.
        • Yang Z.C.
        • Chen L.
        • et al.
        Inhibition airway remodeling and transforming growth factor-β1/Smad signaling pathway by astragalus extract in asthmatic mice.
        Int J Mol Med. 2012; 29: 564-568
        • Bao Z.
        • Lim S.
        • Liao W.
        • et al.
        Glycogen synthase kinase-3β inhibition attenuates asthma in mice.
        Am J Respir Crit Care Med. 2007; 176: 431-438
        • Wong E.L.
        • Sung R.Y.
        • Leung T.F.
        • et al.
        Randomized, double-blind, placebo-controlled trial of herbal therapy for children with asthma.
        J Altern Complement Med. 2009; 15: 1091-1097
        • Li-Weber M.
        • Krammer P.H.
        Regulation of IL4 gene expression by T cells and therapeutic perspectives.
        Nat Rev Immunol. 2003; 3: 534-543
        • Hogan S.P.
        • Rosenberg H.F.
        • Moqbel R.
        • et al.
        Eosinophils: biological properties and role in health and disease.
        Clin Exp Allergy. 2008; 38: 709-750
        • Galli S.J.
        • Tsai M.
        • Piliponsky A.M.
        The development of allergic inflammation.
        Nature. 2008; 454: 445-454
        • Medoff B.D.
        • Thomas S.Y.
        • Luster A.D.
        T cell trafficking in allergic asthma: the ins and outs.
        Annu Rev Immunol. 2008; 26: 205-232
        • Das J.
        • Chen C.H.
        • Yang L.
        • et al.
        A critical role for NF-κB in GATA3 expression and Th2 differentiation in allergic airway inflammation.
        Nat Immunol. 2001; 2: 45-50
        • Bao Z.
        • Guan S.
        • Cheng C.
        • et al.
        A novel antiinflammatory role for andrographolide in asthma via inhibition of the nuclear factor-kappaB pathway.
        Am J Respir Crit Care Med. 2009; 179: 657-665
        • Wang Y.J.
        • Wang J.T.
        • Fan Q.X.
        • et al.
        Andrographolide inhibits NF-κB activation and attenuates neointimal hyperplasia in arterial restenosis.
        Cell Res. 2007; 17: 933-941
        • Pantano C.
        • Ather J.L.
        • Alcorn J.F.
        • et al.
        Nuclear factor-κB activation in airway epithelium induces inflammation and hyperresponsiveness.
        Am J Respir Crit Care Med. 2008; 177: 959-969
        • Klemm S.
        • Ruland J.
        Inflammatory signal transduction from FceRI to NF-κB.
        Immunobiology. 2006; 211: 815-820
        • Schulze-Luehrmann J.
        • Ghosh S.
        Antigen-receptor signaling to nuclear factor κB.
        Immunity. 2006; 25: 701-715
        • Desmet C.
        • Gosset P.
        • Pajak B.
        • et al.
        Selective blockade of NF-κB activity in airway immune cells inhibits the effector phase of experimental asthma.
        J Immunol. 2004; 173: 5766-5775